ARL Group ARL Group

New Methods – Thermophilic Amoeba

Experience

Thermophilic Amoeba and Naegleria (including speciation)

ARL Group is now NATA accredited for Thermophilic Amoeba and Naegleria (including Naegleria fowleri) analysis in Waters.

Naegleria fowleri (also known as the “brain-eating amoeba”) is a free-living, thermophilic unicellular organism.

Thermophilic Amoeba and Naegleria thrive at relatively high temperatures – up to 46°C. Any water body that seasonally exceeds 30°C or continually exceeds 25°C can support growth of this organism; typically warm bodies of fresh water, such as ponds, lakes, rivers, and hot springs. Although lower temperatures are not advantageous for activity and growth, the Amoeba can occur as a dormant cyst, waiting for favorable conditions to return.

Naegleria fowleri is currently the only type of Naegleria known to infect humans. It enters the body via the nasal passage where it travels up to the brain causing inflammation that destroys the brain tissue. Although rare, this infection (known as Primary Amoebic Meningoencephalitis – PAM) is usually fatal.


Testing Areas

The presence of Thermophilic Amoeba and Naegleria is confirmed by microscopy, PCR and qPCR; which are molecular methods for confirmation. This methodology can also allow for the optional barcoding of other Naegleria sp. if required.

  • Bottle Requirements
    • A minimum of 250mL is required is a sterile sodium thiosulphate preserved plastic bottle.
    • This needs to be keep at an ambient temperature (Not Chilled)
  • Holding Time
    • Recommended holding time of 24 hours. Up to 96 hours is considered acceptable as per AS2031:2012.
  • Reporting
    • Detected/Not Detected in 250mL

Analysis

  • Water

Water

Thermophilic Amoeba and Naegleria (including speciation)